![](https://parts.igem.org/images/partbypart/icon_generator.png)
Generator
Part:BBa_K2014010:Design
Designed by: Daria Niewiadomska Group: iGEM16_UAM_Poznan (2016-10-12)
pxylS-E1_5'UTR->sfGFP_W
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 203
Illegal SpeI site found at 338
Illegal SpeI site found at 515 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 203
Illegal SpeI site found at 338
Illegal SpeI site found at 515 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 203
Illegal SpeI site found at 338
Illegal SpeI site found at 515 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 203
Illegal SpeI site found at 338
Illegal SpeI site found at 515 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We were looking for the best method to optimize gene expression in E.coli.
Source
We used one of the sfGFP permutation, with optimized codon sequence.